Susceptibility of Chlamydia trachomatis to chlorhexidine gluconate gel.
نویسندگان
چکیده
To identify topical antimicrobial preparations which may be effective in preventing the transmission of sexually transmitted diseases, we examined the activity of chlorhexidine gluconate (CHG) against Chlamydia trachomatis. Chlamydial elementary bodies were incubated with dilutions of CHG gel for various times from 0 to 120 min. An aliquot of each dilution was further diluted and was inoculated onto McCoy cell monolayers in individual wells in a 96-well microtiter plate. The cultures were incubated for 48 h, and the chlamydial inclusions were stained and counted. CHG gel diluted fourfold (0.0625% CHG) killed C. trachomatis serovar D, and CHG gel diluted eightfold (0.0313% CHG) killed serovar F immediately upon exposure. CHG gel diluted 16-fold (0.0156% CHG) killed serovar D, and CHG gel diluted 32-fold (0.0078% CHG) killed serovar F after 120 min of exposure. Alteration of the pH over the range of from 4 to 8 did not significantly affect its activity. The addition of 10% whole human blood decreased the CHG gel activity at 0 min but had no significant effect after 120 min of exposure. We conclude that CHG gel may be effective topically against C. trachomatis at concentrations that can be used and under conditions that are found in the female genital tract and that further studies of its antimicrobial efficacy and toxicity in vivo are warranted.
منابع مشابه
P-41: Association Study of MICA*008 Gene Polymorphism with Chlamydia Trachomatis Infection in Infertile Men Reffer to Royan Institute
Background: Chlamydia trachomatis(CT) is an obligate intracellular bacteria, requires living cells to replicate itself. CT infection can remain up to 4 years in the couple and affect their fertility. The relationship between CT and infertility is very important because most patients are asymptomatic and untreated. After infection with CT, NK activation signals begin through interactions of its ...
متن کاملComparative study of the antimicrobial efficacy of chlorhexidine gel, chlorhexidine solution and sodium hypochlorite as endodontic irrigants.
The purpose of this study was to assess the in vitro the antimicrobial efficacy of chlorhexidine gluconate gel as an endodontic auxiliary chemical substance compared to sodium hypochlorite (NaOCl) and chlorhexidine gluconate solution. The antimicrobial efficacy of the tested substances was evaluated using the agar diffusion test. The growth inhibition zones produced by 0.2%, 1% and 2% chlorhexi...
متن کاملCloning, Expression and Purification of Truncated Chlamydia Trachomatis Outer Membrane Protein 2 (Omp2) and its Application in an ELISA Assay
Background: Although a simple and direct method does not exist for the detection of chlamydial infections, there are situations in which reliable serological tests, with sensi-tivity related to a specific antigen, could be helpful. Objective: The aim of this study was to clone the first 1100 bp of the C. trachomatis outer membrane protein 2 (omp2) gene in order to prepare a recombinant protein ...
متن کاملP-59: Chlamydia Trachomatis Infection in Femaleswith Secondary Infertility
Background: We designed a prospective study to assess the role of Chlamydia in secondary infertility Materials and Methods: 40 women with secondary infertility and 30 term healthy pregnant women of similar age composition were studied for past and present chlamydia trachomatis infection. Women attending the outpatient department of Obstetrics and Gynaecology with complaint of secondary infertil...
متن کاملAnti-Chlamydia trachomatis antibodies and abortion in women referring to
Introduction: Fetal loss and abortion are responsible for significant emotional distress for couples desiring children. Regarding the etiologic importance, this study has been carried out to determine the prevalence of Anti-Chlamydia trachomatis antibodies in normal women and women with abortion. Methods: In this descriptive study 120 women with definite diagnosis of abortion as patients and ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Antimicrobial agents and chemotherapy
دوره 42 7 شماره
صفحات -
تاریخ انتشار 1998